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| Jiang,Guo-Feng; Jiang,Bo-Le; Yang,Mei; Liu,San; Liu,Jiao; Liang,Xiao-Xia; Bai,Xian-Fang; Tang,Dong-Jie; Lu,Guang-Tao; He,Yong-Qiang; Yu,Di-Qiu; Tang,Ji-Liang. |
| It is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector expression and secretion. Xanthomonas campestris pv. campestris (Xcc) is a model bacterium for studying plant-pathogen interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME, MMX, XVM2, and XOM2) which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most efficient for expression... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inducing medium; Type III effector secretion; Xanthomonas. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045 |
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| Yang,Shaofeng; Sheng,Nan; Pan,Lili; Cao,Jing; Liu,Jiao; Ma,Ran. |
| Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MicroRNA-3129; Gastric cancer; PRb; Proliferation; Cell cycle. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600612 |
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